Signalling, Nuclei and Innovations in Oncology
In cells, interdependent structures function through dynamic processes that rely on many complex mechanisms. Perturbations of these mechanisms occurring in normal cells can result in various types of pathologies such as genetic or epigenetic diseases and cancers. The scientific goals of the UMR 8126 follow two main axes :
- We are aiming at better understanding the most important functions that take place in the cell nucleus: DNA replication, repair and recombination, chromatin remodelling and chromosome reorganisation. More specifically, our teams analyse nucleoprotein complexes by molecular and cellular imaging procedures, study the 3D organisation of the nucleus and develop tools for proteomics to decipher how the epigenetic information is encoded and modified at the level of chromatin.
- We investigate how various signalling pathways are involved in oncogenetic processes that take place both within cells and between a tumour and its host. Current investigations concern:
- proteins encoded by an oncogenic herpesvirus, the Epstein-Barr virus (EBV), that infects B lymphocytes and epithelial cells;
- the p53 and Nprl2 tumour suppressor proteins;
- miRNAs of specific interest;
- the role of exosomes and other nano-objects produced by tumour cells;
- the metastatic process referred to as “collective invasion”.
These knowledge-oriented investigations rely upon the use of clinical material either kept and propagated in vitro in the laboratory or directly obtained from tumour biopsies. They originate from patients with haematological malignancies (Mantle Cell Lymphoma, Burkitt’s Lymphoma and Post-Transplant Lymphoproliferative Disorders) or solid tumours (Nasopharyngeal Carcinoma, Ovarian Carcinoma, Neuroblastoma, Colorectal Cancer) and also from patients with Facio-Scapulo-Humeral Muscular Dystrophy, a genetic disease of particular interest.
The UMR 8126 comprises 7 teams:
- Intracellular traffic, macromolecular complexes and cancer
- Proteomics and epigenetics
- Nuclear organisation and pathological models
- Oncogenesis and resistance to apoptosis in B cell lymphomas
- Genome Maintenance, Molecular Microscopies and Bionanosciences
- Tumour microenvironment, exosomes and microRNAs in solid tumours
- Collective invasion and epithelial morphogenesis