Molecular biopathology

Molecular biopathology section

This section performs molecular assays (sequencing of more than 350 targets, PCR, qPCR, NGS etc.) in the context of clinical trials or research studies. This section is also involved in testing for mutations in the context of the Gustave Roussy Personalised Medicine programme.

The skill base of the section has been broadened by developing a team to research knowledge transfer in Haematology.
The nature of blood diseases has led to the development of methods of cell selection designed to isolate sub-populations from tumour samples.

In 2012 the laboratory established a new generation IonTorrent® approach to sequencing, which now has applications in all of the projects involving molecular biology assays.

• Analysis of samples required for the inclusion of patients in therapeutic trials (targeted analysis),
• Ancillary study and translational research project in academic or commercial collaboration (s-Hemo project with sequencing study in the chronic hematological malignancies),
• Mutation studies in Personalised Medicine programmes (MSN, MOSCATO, MSN, SAFIR [trial of personalised treatment in breast cancer]…),
• Development of high-throughput sequencing analysis in clinical research studies (IonTorrent®/PGM®)
• R&D work focusing on the detection of mutations in circulating tumour DNA.
• Introduction of work with Affymetrix® DNA gene chips (SNP array…)

The laboratory has carried ISO9001 certification since 1st July 2014

H2 Molecular biopathology equipment

• 2 2100 Bioanalysers (Agilent®)
• 1 NanoDrop® for sample quantification (Ozyme®)
• 1 Liquidator (Mettler Toledo®)
• 2 QUBIT® sample quantification (Labtech®)
• 6 PCR blocks (96 wells)
• 1 ViiA7 PCR Quantification block, 96 wells (Life Technologies®)
• 2 PCR VERITI® blocks (Life Technologies®)
• 1 DNA Extractor EZ1 (Qiagen®)
• 1 48 Capillary array sequencer shared with Biopath (Life Technologies®)
• 2 PGM IonTorrent® + 3 OneTouch, NG sequencing (Life Technologies®)
• 1 Affymetrix® Gene Chip Platform
• AutoMACS® Pro Separator (Miltenyi Biotec®)

Team members

• Lacroix Ludovic, Biologist/Team leader
• Jacques Nathalie, Laboratory manager
• Saulnier Patrick, Biologist engineer
• Jovelet Cécile, Postdoctoral researcher
• Camara-Clayette Valérie, Biologist engineer 
• Honoré Aurélie, Technician
• Laporte Mélanie, Technician 
• Lecourt Hélène, Technician
• Louis Maxime, Technician
• Motté Nelly, Technician
• Paume Clélia, Technician
• Richon Catherine, Technician