This platform is attached to the Integrated Biology Platform
The Gustave Roussy genomics platform provides a molecular biology service in the fields of transcriptomics and genomics for all basic and translational research projects conducted in the context of academic collaboration and work in conjunction with French and international companies. The genomics department offers help with study design and the preparation and/or validation of biological specimens (RNA/DNA) extraction the performance of microarray experiments (CGH, GE et miRNA), TLDA PCR arrays, TLDA microRNA PCR assays and provides the latest high-throughput technology for nucleic acid sequencing. It also engages with research on mutations and the genotype. By virtue of the SIRIC SOCRATE programme, new cutting-edge apparatus has been acquired (MiSeq Illumina sequencer, Covaris E220 LGC genomics robot). This has helped in the development of techniques of WGS (Whole Genome Sequencing), WES (Whole Exome Sequencing), RNAseq (transcriptom), ChIPseq (Chromatin Immuno-Precipitation) and of a protocol of Ion AmpliSeq on MiSeq
The department works closely with the Bioinformatics Platform
- Elaboration of optimal experimental design in collaboration with workers on projects
- Advice on design and initiation of projects
- Advice and help in preparing samples and in quality control.
- Production of microarrays Agilent (Gene Expression, miRNA, Comparative Genomics Hybridisation)
- High throughput sequencing using Ion Torrent technologies (Life Technologies) and Illumina (HiSeq2000 et MiSeq) methods
- Provision, with conditions, of the following apparatus: Nanodrop (quantification of nucleic acids), Bioanalyser (quality control of nucleic acids or proteins), real-time PCR apparatus (96-well plates or TLDA cards), Covaris S220 (DNA fragmentation)
- Acquisition of a Biomark with a C1 module (Fluidigm) (development of single cell protocols: Taqman nano-analyses (SNP or real-time PCR) for simultaneous, rapid analysis of 48 or 96 samples from 48 or 96 targets (SNP or genes, up to 9216 simultaneous PCR); RNA-seq; AmpliSeq on MiSeq established in the department enabling the study of mutations with samples from as little as 10ng.