Imaging and Cytometry Platform (PFIC)

Head of platform
Dr. Corinne Laplace-Builhé
Tel: + 33 (0)1 42 11 66 72

Pavillon de recherche 2, 1st floor

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Imaging and Cytometry Platform (PFIC)

Imaging and Cytometry Platform

The PFIC sits at the interface between basic, translational and clinical research. It functions both as a department offering services and as a research and development unit. 

It makes its expertise available to Gustave Roussy research teams, academic teams from outside Gustave Roussy and to industry. It provides special skills in flow cytometry, cell sorting, photon imaging, multiphoton molecular and cellular imaging, tissue imaging and small animal photon imaging.    

Governance and management

The Gustave Roussy scientific directorate is responsible for governance of the PFIC and defines its strategy.

A mangement committee consisting of 10 researchers/users and departmental engineers meets once or twice per year to determine the major lines of development of the department in terms of its functioning and apparatus.   

R&D work in imaging is carried out in team 3 «Multimodality imaging in oncology» of UMR 8081-CNRS- IR4M 

Services provided

The PFIC provides services in photon imaging and imaging and flow cytometry. It trains users in various biomedical imaging fields and in flow cytometry. The PFIC engineers help users during image acquisition and advise them on the handling and interpretation of the data obtained, as well as the conduct of flow and imaging cytometry experiments. The PFIC works with research teams to develop new methods of sample testing. It also provides essential methodological and technical developments to enable new scientific pathways to be opened up.  

Researchers from outside Gustave Roussy, both academic and from industry 

Access to apparatus for researchers from outside Gustave Roussy, cost estimates for training courses and prices for services may be obtained by approaching the individuals in technical charge of the equipment.     
Development work requested by research units outside Gustave Roussy (other than collaboration), require the submission of a project summary by the applicant to the PFIC head. The project will be allowed to start only after assessment and approval by the PFIC scientific committee.   

Research and development

High resolution imaging in vivo and transfer of photon imaging to clinical practice

By ensuring continuity between basic research and clinical application of photon imaging, the PFIC is specifically committed to and sponsor of several pre-clinical and clinical programmes which focus on development and transfer into practice of non-invasive high resolution imaging in patients and small animals.

Preclinical projects
  • High resolution imaging in the small animal – development  of dorsal window chambers for multimodality imaging  
  • Non-invasive optical biopsies for high resolution evaluation of cancer using full-field optical coherence tomography (European CAReIOCA – FP7 project)
  • PERSEE project: Robotic Endomicroscopy in the redefinition of Excision Strategies (OSEO-ISI /BPI)
Clinical trial

Clinical assessment of  endoscopic confocal microscopy in ENT oncology – (MEC ORL: Confocal Micro- Endoscopy in ENT)  

Training

Training of students and researchers in the techniques of cytometry and imaging is a major function of the PFIC. In general, it trains, on average, more than a hundred researchers per year taking all the techniques of photon imaging and cytometry together. This training involves both the underlying theoretical basis and practical work on optical imaging and flow cytometry apparatus.  
In 2014 a total of 418 hours of training in imagery and cytometry was provided by the department to on-site Gustave Roussy researchers and to external teams, as well as to students obtaining masters qualifications:  

Teaching on photon imaging 

  • M2  Biotechnology (University of Orléans)
  • M2 Medical Physics – Methodology-instrumentation & Applications in Clinical Practice modules (University of Paris South 11)
  • M 2 Biology of Health  - Specialist Oncology (University of Paris South 11)

Teaching on flow cytometry and cell sorting 

  •  M2 Engineering in Biology (University of Paris V - Descartes) 
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