Microscopy - recent publications

• Endothelial and hematopoietic hPSCs differentiation via a hematoendothelial progenitor Alejandra Vargas‑Valderrama, Anne‑Charlotte Ponsen, Morgane Le Gall, Denis Clay, Sébastien Jacques, Tudor Manoliu, Valérie Rouffiac, Karine Ser‑le‑Roux, Cyril Quivoron, Fawzia Louache, Georges Uzan, Maria‑Teresa Mitjavila‑Garcia, Estelle Oberlin1† and Hind Guenou

Microscopy - Equipments

SP8 Confocal Microscope (Leica)

The system in reverse configuration is equipped with 4 lasers covering excitation wavelengths 405, 488, 561, and 638nm. Fluorescence signals are collected on 2 PMT detectors and 2 high-sensitivity HyD detectors. A thermostatic and CO2-regulated chamber and a motorized turntable are installed on the microscope for kinetic studies and XYZT acquisitions.

This microscope is suitable for confocal, wide-field and/or multi-position imaging.

Microscopy - Activities

• Theoretical classes and practical training in photonic microscopy technics   
• Confocal and multiphoton imaging of cells and tissues
• TIRF, live-SR, FRAP, FRET, Photoconversion, Optogenetics
• Multidimensional dynamic imaging in living organs & 3D organoid models
• Support for the development of new optical devices
• Design of experimental procedures
• Scientific animations
• Technological developments and innovation
• Imaging of organoid models

Cytometry & Mass imaging - Team members

Operational manager:
Philippe Rameau (Research Engineer)
Tel. : +33 (0)1.42.11.51.38
philippe.rameau@gustaveroussy.fr

Cyril Catelain (Research Engineer)
Tel. : +33 (0)1.42.11.51.38
cyril.catelain@gustaveroussy.fr

Cytometry & Mass imaging - Publications

• Low Doses of Radiation Increase the Immunosuppressive Profile of Lung Macrophages During Viral Infection and Pneumonia Meziani L, Robert C, Classe M, Da Costa B, Mondini M, Clémenson C, Alfaro A, Mordant P, Ammari S, Le Goffic R, Deutsch E.. Int J Radiat Oncol Biol Phys. 2021 Aug 1;110(5):1283-1294. doi: 10.1016/j.ijrobp.2021.03.022. Epub 2021 Mar 13. PMID: 33722770; PMCID:PMC7954779.

Cytometry & Mass imaging - Equipments

Mass cytometer HELIOS (Fluidigm)

As a complementary tool to flow cytometers, the mass cytometer considerably increases the number of parameters that can be analyzed (>40), while eliminating the problems of overlapping fluorescence spectrums observed with conventional cytometry.

Cytometry & Mass imaging - Activities

Training and coaching

• Flow, spectral and mass cytometry
• Cell sorting and single-cell isolation
• Mass imaging for molecular, cellular and functional spatial mapping of cells and tissues
• Bioinformatics adapted to the unsupervised analysis of cytometry and imaging data.

Design of experimental procedures