Cytometry and Imaging - Microscopy : equipment

Microscopy - Equipments

SP8 Confocal Microscope (Leica)

The system in reverse configuration is equipped with 4 lasers covering excitation wavelengths 405, 488, 561, and 638nm. Fluorescence signals are collected on 2 PMT detectors and 2 high-sensitivity HyD detectors. A thermostatic and CO2-regulated chamber and a motorized turntable are installed on the microscope for kinetic studies and XYZT acquisitions.

This microscope is suitable for confocal, wide-field and/or multi-position imaging.

Cytometry and Imaging - Microscopy : Activities

Microscopy - Activities

  • Theoretical classes and practical training in photonic microscopy technics   
  • Confocal and multiphoton imaging of cells and tissues
  • TIRF, live-SR, FRAP, FRET, Photoconversion, Optogenetics
  • Multidimensional dynamic imaging in living organs & 3D organoid models
  • Support for the development of new optical devices
  • Design of experimental procedures
  • Scientific animations
  • Technological developments and innovation
  • Imaging of organoid models

Cytometry & Mass imaging - Team members

Cytometry & Mass imaging - Team members

Operational manager:
Philippe Rameau (Research Engineer)
Tel. 01.42.11.51.38
philippe.rameau@gustaveroussy.fr

Cyril Catelain (Research Engineer)
Tel. 01.42.11.51.38
cyril.catelain@gustaveroussy.fr

Cytometry & Mass imaging - Publications

Cytometry & Mass imaging - Publications

  • Low Doses of Radiation Increase the Immunosuppressive Profile of Lung Macrophages During Viral Infection and Pneumonia Meziani L, Robert C, Classe M, Da Costa B, Mondini M, Clémenson C, Alfaro A, Mordant P, Ammari S, Le Goffic R, Deutsch E.. Int J Radiat Oncol Biol Phys. 2021 Aug 1;110(5):1283-1294. doi: 10.1016/j.ijrobp.2021.03.022. Epub 2021 Mar 13. PMID: 33722770; PMCID:PMC7954779.

    Cytometry & Mass imaging - Equipments

    Cytometry & Mass imaging - Equipments

    Mass cytometer HELIOS (Fluidigm)

    As a complementary tool to flow cytometers, the mass cytometer considerably increases the number of parameters that can be analyzed (>40), while eliminating the problems of overlapping fluorescence spectrums observed with conventional cytometry.

    Cytometry and Mass imaging - Activities

    Cytometry & Mass imaging - Activities

    Training and coaching

    • Flow, spectral and mass cytometry
    • Cell sorting and single-cell isolation
    • Mass imaging for molecular, cellular and functional spatial mapping of cells and tissues
    • Bioinformatics adapted to the unsupervised analysis of cytometry and imaging data.

    Design of experimental procedures

    La recherche en immunothérapie

    Premier centre d’immunothérapie en Europe, Gustave Roussy poursuit sa stratégie de développement des immunothérapies et lance un programme médico-scientifique pour mieux comprendre leurs toxicités afin de les prévenir et ainsi améliorer la prise en charge des patients.

    Dans la lancée de son précédent programme d’immunothérapie GRIP, initié en 2015, Gustave Roussy fédère les différentes disciplines et expertises de ses équipes pour accélérer la recherche en immunothérapie avec un volet spécifique dédié à la prise en charge de ses effets secondaires.   

    Prism

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